Comparison of Fully Porous and Superficially Porous Particles for the Analysis of Synthetic Oligonucleotides

Abstract:

Superficially porous particle technologies provide a reduced diffusion path in comparison with their fully porous counterparts reducing the resistance to mass transfer during chromatographic separation. With these particles, one can benefit from their greater efficiency either through improved separation or achieving comparable efficiency with a larger particle size. This enables the method flexibility to increase flow rate . When this is coupled with the benefits of bio-inert hardware, a greater level of separation of oligonucleotide impurities can be observed compared to a sub -2µm fully porous hybrid silica. The potential to implement a faster flow rate offers the opportunity for shorter analysis times or increased sample throughput ; further lower backpressure can contribute to improved column lifetime especially considering the harsh conditions associated to oligo analysis by ion-pairing reversed phase LC.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

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